*Metadata Recording: Recording quality: [ fair, fair, poor, good, ok, bad ] Comment : "relatively low spikes, hard to find the right number of channels" Experimenter : [ Paul Pfeiffer, Paul Pfeiffer, Me, You ] Temperature : 34 ElectrodeProgram : 14 Cell: Cell type : [ pyramidal cell, pyramidal cell, Wang-Buzaki, unknown, I, E ] Location (Recording location): [ PR LII, PR LII, CMS, MS, CLS, LS, ~ ] Depth : 0um Subject: Species : [ Mouse, A. leptorhynchus, passive cell model, Rat, Mouse ] Sex : [ unknown, Male, Female, unknown ] Preparation: Slice *Control: SpectrumAnalyzer intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] origin : [ before end of data, before signal, after signal ] offset : 0ms duration : 1000ms resolution: 10Hz overlap : true window : [ Hanning, Bartlett, Blackman, Blackman-Harris, Hamming, Hanning, Parzen, Square, Welch ] fmax : 5000Hz decibel : true pmin : -50dB *Model: NeuronModels Spike generator: spikemodel: [ Wang-Buzsaki, Stimulus, Passive membrane, Morris-Lecar, Hodgkin-Huxley, Connor, Wang-Buzsaki ] noised : 0.005 deltat : 0.01ms integrator: [ Euler, Midpoint, Runge-Kutta 4 ] Voltage-gated current 1 - activation only: gmc : 0.5 emc : -90mV mvmc : -35mV mwmc : 10mV taumc: 100ms Voltage-gated current 2 - activation and inactivation: gmhc : 0 emhc : -90mV mvmhc : -40mV mwmhc : 10mV taummhc: 10ms pmmhc : 1 hvmhc : -40mV hwmhc : 10mV tauhmhc: 10ms pmhhc : 1 *Model: Stimulus Input: gain : 1 offset: 0muA/cm^2 *Model: Passive membrane Parameter: R: 50MOhm C: 25pF Input: gain : 1 offset: 0muA/cm^2 *Model: Morris-Lecar General: params: [ Type I, Custom, Type I, Type II ] Calcium current: gca : 4nS eca : 120mV mvca: -1.2mV mkca: 18mV Potassium current: gk : 8nS ek : -80mV mvk : 12mV mkk : 17.4mV mphik: 0.067kHz Leak current: gl: 2nS el: -60mV c : 20pF Input: timescale: 2 gain : 1 offset : 0muA/cm^2 *Model: Hodgkin-Huxley Sodium current: gna: 120mS/cm^2 ena: 50mV Potassium current: gk: 36mS/cm^2 ek: -77mV Leak current: gl : 0.3mS/cm^2 el : -54.4mV c : 1muF/cm^2 phi: 1 Input: gain : 20 offset: 0muA/cm^2 *Model: Connor Sodium current: gna: 120mS/cm^2 ena: 50mV Potassium current: gk: 20mS/cm^2 ek: -77mV A current: gka: 47mS/cm^2 eka: -80mV Leak current: gl : 0.3mS/cm^2 el : -22mV c : 1muF/cm^2 phi: 1 Input: gain : 20 offset: 0muA/cm^2 *Model: Wang-Buzsaki Sodium current: gna: 35mS/cm^2 ena: 55mV Potassium current: gk: 9mS/cm^2 ek: -90mV Leak current: gl : 0.1mS/cm^2 el : -65mV c : 1muF/cm^2 phi: 5 Input: gain : 5 offset: 0muA/cm^2 *Event Detector: Spikes-1 Detector: threshold: 0.0mV Indicators: log: true *RePro: Pause duration : 1sec savedata : true plotwidth: 1sec *RePro: Record duration : 10sec plotwidth: 0sec dioout : false diodevice: dio-1 dioline : 0 *RePro: SaveTraces General: duration : 10sec savedata : true split : false dioout : false diodevice: dio-1 dioline : 0 Analog input traces: trace-V-1 : true trace-Current-1 : true trace-Open-Channels : true trace-Cooperative-Cluster-Current: true trace-Stimulus-Current-1 : true Events: events-Spikes-1: true *RePro: SetAttenuatorGain outrace : V-1 gain : 1 interactive: true *RePro: SetInputGain intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] gainindex : 0 interactive: false *RePro: SetOutput outtrace : [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] value : 0nA intensity : 1 interactive: false *RePro: Spectrogram intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] width : 100ms step : 0ms tmax : 10s duration: 0s size : [ "1024", "64", "128", "256", "512", "1024", "2048", "4096", "8192", "16384", "32768", "65536", "131072", "262144", "524288", "1048576" ] overlap : true window : [ Hanning, Bartlett, Blackman, Blackman-Harris, Hamming, Hanning, Parzen, Square, Welch ] powermax: true fmax : 2000Hz pmax : 0dB pmin : -50dB *RePro: TransferFunction Stimulus: outtrace : [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] offsetbase: [ custom, current ] offset : 0nA amplitude : 0.05nA clip : 4 intensity : 1 fmin : 0Hz fmax : 100Hz duration : 10s pause : 1s repeats : 100 Analysis: intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] size : [ "1024", "64", "128", "256", "512", "1024", "2048", "4096", "8192", "16384", "32768", "65536", "131072", "262144", "524288", "1048576" ] overlap : true window : [ Hanning, Bartlett, Blackman, Blackman-Harris, Hamming, Hanning, Parzen, Square, Welch ] plotstdevs : false plotcoherence: true plotdecibel : false *RePro: Wait absdate: false date : ~ days : 0days time : "00:00:00.000" *RePro: AttenuatorCheck outtrace : [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] duration : 1s type : [ attenuation, noise ] frequency: 100Hz amplitude: 1Vmax minlevel : 0dB maxlevel : 100dB dlevel : 1dB adjust : true *RePro: InputRangeCheck outtrace: [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] duration: 1000ms pause : 1000ms amplnum : 10 *RePro: RestartDelay duration: 100ms repeats : 100 intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] *RePro: StimulusDelay intrace : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] outtrace : [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] samplerate: 20kHz duration : 10ms pause : 30ms repeats : 1000 setdelay : [ none, minimum, mean ] *RePro: BridgeTest amplitude : -0.3nA duration : 200ms pause : 50ms average : 2 skipspikes : true dynamicrange: true rate : 0.01 plottrace : false *RePro: CalibrateSyncPulse imin : -1nA imax : 1nA istep : 0.001nA skipwin : 1000ms duration: 1000ms *RePro: CapacityCompensation amplitude : 0.5nA duration : 20ms frequency : 3000Hz showcycles : 8 pause : 20ms average : 10 skipspikes : true dynamicrange: false rate : 0.01 *RePro: Iontophoresis durationpos : 1s amplitudepos : 1nA pausepos : 1s durationneg : 1s amplitudeneg : 1nA pauseneg : 1s fortunes : true fortuneperiod: 10s *RePro: SetLeak interactive : true preset : [ previous, zero, custom ] g : 0nS E : 0mV reversaltorest: true involtage : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] duration : 100ms *RePro: SetVGate interactive : true preset : [ previous, zero, custom ] g : 0nS E : 0mV vmid : 0mV width : 0mV tau : 10ms reversaltorest: true involtage : [ V-1, Current-1, Open-Channels, Cooperative-Cluster-Current, Stimulus-Current-1 ] duration : 100ms *RePro: FICurve Stimuli: ibase : [ zero, DC, threshold ] imin : 0nA imax : 1nA istep : 0.1nA optimizedimin: 1000nA manualskip : false Timing: duration : 300ms delay : 100ms pause : 300ms ishuffle : [ Up, Down, AlternateInUp, AlternateInDown, AlternateOutUp, AlternateOutDown, Random, PseudoRandom ] shuffle : [ Up, Down, AlternateInUp, AlternateInDown, AlternateOutUp, AlternateOutDown, Random, PseudoRandom ] iincrement : -1 singlerepeat: 2 blockrepeat : 2 repeats : 1 Analysis: fmax : 100Hz vmax : -50mV numpoints : 0 sswidth : 50ms ignorenoresponse: true diffincrement : 0 maxratediff : 10Hz *RePro: FindRate rate : 10Hz ratetol : 1Hz startamplitudesrc: [ custom, custom, DC, threshold, previous ] startamplitude : 0.45nA amplitudestep : 0.1nA duration : 2000ms skipwin : 100ms *RePro: MembraneResistance Stimulus: amplitude: -0.1nA duration : 200ms pause : 600ms repeats : 0 Analysis: skipspikes : true sswidth : 50ms nossfit : false plotstdev : true setdata : [ rest only, always, never ] checkoutput: Current-1 *RePro: PhaseResettingCurve dcamplitudesrc: [ DC, custom, DC ] dcamplitude : 0.1nA amplitude : 0.2nA duration : 1ms nperiods : 5 repeats : 100 rateduration : 1000ms averageisis : 10test-pulses minsave : 10 *RePro: SetDC outcurrent : Current-1 dcamplitudesel : [ to previous value, to absolute value, to previous value, to a fraction of the threshold, relative to threshold ] dcamplitude : 0nA dcamplitudefrac: 90% dcamplitudedecr: 0nA interactive : true dcamplitudestep: [ "0.01", "0.001", "0.002", "0.005", "0.01", "0.02", "0.05", "0.1", "0.2", "0.5", "1" ] showstep : true duration : 20seconds update : 1seconds showstdev : true *RePro: Simple intrace : V-1 outtrace : [ Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] amplitude : 0.3nA duration : 10ms stimulus : [ sine 1p, constant, ramp, sine 1p, sine 2p, zero ] samerate : true samplerate: 1kHz pause : 10ms repeats : 0 save : false *RePro: SingleStimulus Waveform: waveform : [ Rectangular, From file, Const, Sine, Rectangular, Triangular, Sawup, Sawdown, Alpha, Whitenoise, OUnoise, Sweep ] stimfile : "2016-12-16-ab/Rectangular0.025Hz34000msr0ms0.25nA0.25.dat" stimampl : 0 amplitude : 0.1nA freqsel : [ period, frequency, period, number of periods ] freq : 0.025Hz period : 25000ms numperiods : 1 pulsesel : [ pulse duration, pulse duration, duty-cycle ] pulseduration: 4000ms dutycycle : 50% tau : 1000ms seed : 3 startfreq : 1.00Hz endfreq : 100.00Hz cycleramp : 0ms duration : 1e+05ms ramp : 0ms Stimulus: offset : 0.1nA offsetbase: [ current, absolute, amplitude, current, threshold, previous ] samerate : true samplerate: 1kHz repeats : 1times pause : 1000ms before : 100ms after : 20000ms outtrace : [ Current-1, Current-1, g, E, J, tau, Cluster size, Number of clusters, Vhalf, Initial state ] Offset - search: userate : false rate : 100Hz ratetol : 5Hz offsetstep : 8nA searchrepeats : 2times silentrate : 0Hz maxsilent : 1 skippause : true maxsearch : 1intensities method : [ Bisect, Bisect, Interpolate ] minslope : 4Hz/nA searchduration: 0ms searchpause : 0ms Analysis: skipwin : 100ms sigma : 10ms storevoltage: true plot : [ Firing rate, Current voltage trace, Mean voltage trace, Current and mean voltage trace, Firing rate ] Save stimuli: storemode : [ session, session, repro, custom ] storepath : ~ storelevel: [ all, all, generated, noise, none ] *RePro: VICurve Stimuli: ibase: [ zero, DC, threshold ] imin : -1nA imax : 1nA istep: 0.1nA userm: false vstep: 1mV Timing: duration : 100ms delay : 100ms pause : 400ms ishuffle : [ Up, Down, AlternateInUp, AlternateInDown, AlternateOutUp, AlternateOutDown, Random, PseudoRandom ] shuffle : [ Random, Up, Down, AlternateInUp, AlternateInDown, AlternateOutUp, AlternateOutDown, Random, PseudoRandom ] iincrement : -2 singlerepeat: 1 blockrepeat : 4 repeats : 1 Analysis: vmin : -100mV sswidth : 50ms ton : 10ms plotstdev: true