# HCS demo

# main figure

The main figure is the result figure, what was achieved. In this case we will probably use a infographics:

mutant/treated mice -> video taped 24h ->

## status before:

excel 1h summary data -> excel analysis of summary data (2-5h):

- p-hacking and harking -> false positive
- 20 to 24h of data, all pooled -> variability and false pos/neg
- no circadian clock reseting - false neg.
- hugly graphs

## status after

behavior sequence data -> metadata creation (1-2h) -> run script

- data reset around light off event
- and cut at shortest period present for all mice,
- statistically sound analysis without a priori (PCA, SVM)
- 3D visualisation

- html/pdf Report

- extendable (sequence analysis)

- FAIR data

## Summary

- faster, more powerful
- clean and statistically sound analysis
- FAIR data

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