Clusters of cooperative ion channels enable a membrane potential-based mechanism for short-term memory

This data repository holds the dynamic clamp recordings and analysis scripts to test whether cooperative ion channels could produce persistent activity in perirhinal cortex neurons.

Overview

Recordings

Cells were patchclamped and the dynamic clamp was used to emulate the presence of multiple clusters of cooperative ion channels. in the membrane.Succesive current pulses were used to test for persistent activity and graded persistent activity.

Analysis

The stimulation protocols were categorized according to the number of pulses and whether the pulses were de- or hyperpolarizing. Then recordings with increasing (respectively decreasing) levels of persistent activity were labelled as successful trials.

Structure of the repository

The repository is structured as follows

recordings: each folder in here corresponds to a recording session from the recording software RELACS
metadata: contains a mapping of cells to the above recording sessions and a manually created blacklist of protocols that are excluded from the analysis
scripts: python scripts to read in the recordings, analyse the firing rates and generate a report
out: contains the automatically generated pdf report with an overview of all cells and plots of each stimulation

How to rerun the analysis

Tested in Linux

Install python dependencies e.g. via conda

   conda env create -f environment.yml

Run analysis and generate the report

   make all

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