# LGN-single-unit-recordings-of-awake-fixating-macaques

Single unit data isolated from linear multielectrode arrays while recording in awake fixating monkeys and gratings drifted on the screen


    Here are important definitions of the variables stored in each file:
    Onsets: stimulus onset times from photodiode
    offsets: stimulus offset times from photodiode
    trstart: trial onset time
    trend: trial end time
    presnum: stimulus presentation number
    trial: trial number
    tilt: grating orientation
    sf: stimulus spatial frequency
    contrast: contrast in dominant eye
    fixedc: contrast non dominant eye
    diameter: stimulus diameter
    eye: dominant eye
    oridist: orientation difference of stimuli between eyes
    phase: grating phase at stim onset
    temporal_freq: grating drifting speed in cyc/sec
    xpos: horizontal eye position 
    ypos: vertical eye position
    photo_on: photodiode onset time
    trg_photo: 0
    refresh: monitor refresh rate
    measured_refresh: empirical monitor refresh rate 
    paradigm: paradigm name
    sdftr_chan: convolved single unit activity
    spk_bin_chan: binary data
    wf: waveforms
    clust: cluster label from kilosort
    norm_autocorr: discretized data
    chan: channel label
    
    Methods: 
    All procedures complied with the regulations by the Association for the Assessment and Accreditation of Laboratory     Animal Care (AAALAC), Vanderbilt University’s Institutional Animal Care and Use Committee (IACUC) and National     Institutes of Health (NIH) guidelines.
    
  
    
    Paradigm:
    For each session, data were collected while animals performed visual tasks that rewarded passive fixation while drifting sine-wave gratings of varying contrast were presented to one or both eyes. The gratings had a spatial frequency of 1 cyc/deg and drifted at a temporal frequency of 4 Hz while the animal fixated within a 1-2 deg radius around the fixation cue (0.5 deg) at the center of the screen. Within each recording epoch, gratings maintained a constant orientation with orientation tilts in the range of -45°, 0°, 45°, 60°, 90°, 135°, 180°, and grating size varied from 2° to 8° . The interstimulus interval varied between a median duration of 1.3 s and 2.1 s across sessions, with an overall median duration of 1.7 s. The stimulus appeared from 250 to 500 ms after the fixation cross appeared on the screen. If the monkey fixated on the cross throughout the whole duration of a trial, it received a juice reward. 
    
    Single units were isolated using Kilosort (1).
    
    References:
    1. Pachitariu M, Steinmetz N, Kadir S, Carandini M, and Harris KD. Kilosort: realtime spike-sorting for extracellular electrophysiology with hundreds of channels. bioRxiv 061481, 2016, 10.1101/061481.)