# Morphological reconstructions and electrophysiological recordings of vibration sensitive honeybee neurons belonging to two maturation levels

This repository contains processed electrophysiology and morphology data used for investigating the honeybee interneuron DL-Int-1 responsive to waggle dance vibration signals.

Reference: Kumaraswamy A., Ai H., Kai K., Ikeno H., Wachtler T. (2019) "Adaptations during maturation in an identified honeybee interneuron responsive to waggle dance vibration signals". In: eNeuro, in Press.

Preprint: Kumaraswamy A., Ai H., Kai K., Ikeno H., Wachtler T. (2018) "Adaptations of honeybee auditory interneurons during maturation". In: bioRxiv. DOI: [10.1101/469502](https://doi.org/10.1101/469502) 

## General Notes

1. A list of neuron IDs as well as their associated age classification is provided in the file "Metadata_age.xlsx"
2. Neuron morphology data is stored in [SWC format](http://research.mssm.edu/cnic/swc.html).
3. Electrophyiology data is stored in [NIX format](https://github.com/G-Node/nix/wiki).
4. The morphology of each DL-Int-1 are represented by multiple SWC files. Each file contains a subregion of DL-Int-1 (see the reference above for description):

    - WN: Whole Arborization
    - MB: Main Branch
    - DB: Dorsal Branch
    - VB: Ventral Branch
   

### Folder Specific Notes

#### ElectrophysiologyData: 
Contains membrane potential and vibration stimulus traces in [NIX format](https://github.com/G-Node/nix/wiki). They data in them can be accessed using the library [GJEphys](https://github.com/wachtlerlab/GJEphys) (see [Usage Guidelines](https://github.com/wachtlerlabGJEphys/doc/Description_Usage.docx )).

#### MorphologyData/MorphologiesBeforeRegistration
Contains DL-Int-1 morphologies reconstructed from LSM images of single dye filled neurons using [Ikeno et al 2018](https://doi.org/10.3389/fninf.2018.00061).

#### MorphologyData/MorphologiesAfterRegistration
Contains DL-Int-1 morphologies after spatial registration using [Reg-MaxS-N](https://doi.org/10.1186/s12859-018-2136-z). Each subfolder contains morphologies using a specific pair of DL-Int-1 morphologies as templates, which is encoded in the name of the folder. A suffix "_xpcScaled" indicates that newly emerged adult morphologies were scaled up by x% before registering them with forager morphologies.