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+Accurate non-linear registration of cortical and subcortical labelling from Mindboggle 101 to the symmetric MNI-ICBM2009c atlas followed by manual editing.
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+
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+ants_nl_registration.sh
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+
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+#! /bin/bash
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+
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+if [ $# -eq 3 ];then
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+ src=$1
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+ trg=$2
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+ outp=$3
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+elif [ $# -eq 5 ];then
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+ src=$1
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+ trg=$2
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+ src_mask=$3
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+ trg_mask=$4
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+ outp=$5
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+else
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+ echo "Usage $0 <source> <target> [source mask] [target mask] <output_prefix>"
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+ echo "Output will be <output_prefix>0_NL.xfm and <output_prefix>0NL_inverse.xfm with corresponding grid files"
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+ exit 1
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+fi
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+
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+if [ ! -z $trg_mask ];then
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+mask="-x [${src_mask},${trg_mask}] "
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+fi
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+
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+
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+antsRegistration -v -d 3 --float 1 \
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+ --output "[${outp}]" \
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+ --use-histogram-matching 0 --winsorize-image-intensities "[0.005,0.995]" \
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+ --transform "SyN[0.7,3,0]" \
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+ --metric "CC[${src},${trg},1,4]" \
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+ --convergence "[50x50x30,1e-6,10]" \
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+ --shrink-factors 4x2x1 --smoothing-sigmas 2x1x0vox \
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+ ${mask} --minc
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+bestlinreg_s2
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+
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+#! /usr/bin/env perl
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+#
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+# linear fitting using parameters optimised by Claude Lepage,
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+# using a brain mask for the source and the target. This was
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+# greatly inspired by best1stepnlreg.pl by Steve Robbins.
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+#
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+# Claude Lepage - claude@bic.mni.mcgill.ca
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+# Andrew Janke - rotor@cmr.uq.edu.au
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+# Center for Magnetic Resonance
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+# The University of Queensland
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+# http://www.cmr.uq.edu.au/~rotor
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+#
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+# Copyright Andrew Janke, The University of Queensland.
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+# Permission to use, copy, modify, and distribute this software and its
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+# documentation for any purpose and without fee is hereby granted,
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+# provided that the above copyright notice appear in all copies. The
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+# author and the University of Queensland make no representations about the
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+# suitability of this software for any purpose. It is provided "as is"
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+# without express or implied warranty.
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+
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+use strict;
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+use warnings "all";
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+use Getopt::Tabular;
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+use File::Basename;
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+use File::Temp qw/ tempdir /;
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+
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+my @conf = (
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+
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+ { type => "blur",
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+ trans => [qw/-est_translations/],
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+ blur_fwhm => 16,
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+ steps => [qw/8 8 8/],
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+ tolerance => 0.01,
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+ simplex => 32 },
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+
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+ { type => "blur",
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+ trans => undef,
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+ blur_fwhm => 8,
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+ steps => [qw/4 4 4/],
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+ tolerance => 0.004,
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+ simplex => 16 },
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+
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+ { type => "blur",
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+ trans => undef,
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+ blur_fwhm => 4,
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+ steps => [qw/4 4 4/],
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+ tolerance => 0.004,
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+ simplex => 8 },
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+
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+ { type => "dxyz",
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+ trans => undef,
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+ blur_fwhm => 8,
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+ steps => [qw/4 4 4/],
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+ tolerance => 0.004,
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+ simplex => 4 },
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+
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+ { type => "dxyz",
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+ trans => undef,
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+ blur_fwhm => 4,
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+ steps => [qw/4 4 4/],
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+ tolerance => 0.004,
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+ simplex => 2 }
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+
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+ );
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+
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+
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+my($Help, $Usage, $me);
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+my(@opt_table, %opt, $source, $target, $outxfm, $outfile, @args, $tmpdir);
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+
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+$me = &basename($0);
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+%opt = (
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+ 'verbose' => 1,
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+ 'clobber' => 0,
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+ 'fake' => 0,
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+ 'init_xfm' => undef,
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+ 'source_mask' => undef,
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+ 'target_mask' => undef,
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+ 'lsqtype' => "-lsq9",
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+ 'work_dir' => undef,
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+ 'sec_source' => undef,
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+ 'sec_target' => undef,
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+ 'cost_function' => '-xcorr',
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+ 'noshear' => 0,
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+ 'noscale' => 0,
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+ 'norot' => 0,
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+ 'noshift' => 0,
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+ 'close' => 0,
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+ );
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+
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+$Help = <<HELP;
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+| $me does hierachial linear fitting between two files.
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+| you will have to edit the script itself to modify the
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+| fitting levels themselves
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+|
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+| Problems or comments should be sent to: claude\@bic.mni.mcgill.ca
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+HELP
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+
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+$Usage = "Usage: $me [options] source.mnc target.mnc output.xfm [output.mnc]\n".
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+ " $me -help to list options\n\n";
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+
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+@opt_table = (
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+ ["-verbose", "boolean", 0, \$opt{verbose},
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+ "be verbose" ],
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+ ["-clobber", "boolean", 0, \$opt{clobber},
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+ "clobber existing check files" ],
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+ ["-fake", "boolean", 0, \$opt{fake},
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+ "do a dry run, (echo cmds only)" ],
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+ ["-init_xfm", "string", 1, \$opt{init_xfm},
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+ "initial transformation (default identity)" ],
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+ ["-source_mask", "string", 1, \$opt{source_mask},
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+ "source mask to use during fitting" ],
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+ ["-target_mask", "string", 1, \$opt{target_mask},
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+ "target mask to use during fitting" ],
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+ ["-lsq9", "const", "-lsq9", \$opt{lsqtype},
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+ "use 9-parameter transformation (default)" ],
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+ ["-lsq12", "const", "-lsq12", \$opt{lsqtype},
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+ "use 12-parameter transformation (default -lsq9)" ],
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+ ["-lsq6", "const", "-lsq6", \$opt{lsqtype},
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+ "use 6-parameter transformation" ],
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+ ["-lsq7", "const", "-lsq7", \$opt{lsqtype},
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+ "use 7-parameter transformation" ],
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+ ["-quaternions","const",'-quaternions', \$opt{lsqtype},
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+ "use quaternion transformation" ],
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+ ["-mi","const",'-mi', \$opt{cost_function},
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+ "Mutual information cost function" ],
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+ ["-xcorr","const",'-xcorr', \$opt{cost_function},
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+ "Cross-correlation cost function" ],
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+ ["-work_dir", "string", 1, \$opt{work_dir},
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+ "Directory to keep blurred files" ],
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+ ["-sec_source", "string", 1, \$opt{sec_source},
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+ "secondaty source" ],
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+ ["-sec_target", "string", 1, \$opt{sec_target},
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+ "secondary target" ],
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+ ["-noshear", "boolean", 1, \$opt{noshear},
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+ "lsq12 w/o shear" ],
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+ ["-noscale", "boolean", 1, \$opt{noscale},
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+ "lsq12 w/o scale" ],
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+ ["-norot", "boolean", 1, \$opt{norot},
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+ "lsq12 w/o rotations" ],
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+ ["-noshift", "boolean", 1, \$opt{noshift},
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+ "lsq12 w/o shifts" ],
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+ ["-close", "boolean", 1, \$opt{close},
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+ "Samples are close" ],
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+ );
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+
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+delete $ENV{MINC_COMPRESS} if $ENV{MINC_COMPRESS};
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+
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+# Check arguments
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+&Getopt::Tabular::SetHelp($Help, $Usage);
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+&GetOptions (\@opt_table, \@ARGV) || exit 1;
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+die $Usage if(! ($#ARGV == 2 || $#ARGV == 3));
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+$source = shift(@ARGV);
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+$target = shift(@ARGV);
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+$outxfm = shift(@ARGV);
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+$outfile = (defined($ARGV[0])) ? shift(@ARGV) : undef;
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+
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+# check for files
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+die "$me: Couldn't find input file: $source\n\n" if (!-e $source);
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+die "$me: Couldn't find input file: $target\n\n" if (!-e $target);
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+if(-e $outxfm && !$opt{clobber}){
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+ die "$me: $outxfm exists, -clobber to overwrite\n\n";
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+ }
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+if(defined($outfile) && -e $outfile && !$opt{clobber}){
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+ die "$me: $outfile exists, -clobber to overwrite\n\n";
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+ }
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+
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+# make tmpdir
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+$tmpdir = &tempdir( "$me-XXXXXXXX", TMPDIR => 1, CLEANUP => 1 );
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+$opt{work_dir}=$tmpdir unless $opt{work_dir};
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+
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+# set up filename base
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+my($i, $s_base, $t_base, $tmp_xfm, $tmp_source, $tmp_target, $prev_xfm,
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+$tmp_sec_source, $tmp_sec_target);
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+
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+$s_base = &basename($source);
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+$s_base =~ s/\.mnc(.gz)?$//;
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+$s_base = 's_'.$s_base;
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+
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+$t_base = &basename($target);
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+$t_base =~ s/\.mnc(.gz)?$//;
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+$t_base = 't_'.$t_base;
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+
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+# Mask the source and target once before blurring. Both masks must exist.
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+
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+my $source_masked = $source;
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+my $target_masked = $target;
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+
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+# initial transformation supplied by the user, applied to both the
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+# source image and its mask.
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+
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+#if( defined $opt{init_xfm} ) {
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+# my $source_resampled = "${tmpdir}/${s_base}_resampled.mnc";
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+# &do_cmd( 'mincresample',
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+# '-clobber',
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+# '-like', $target_masked,
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+# '-transform', $opt{init_xfm},
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+# $source_masked, $source_resampled );
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+# $source_masked = $source_resampled;
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+
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+# #apply it to the mask, if it's defined
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+# if(defined($opt{source_mask}))
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+# {
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+# my $mask_resampled = "${tmpdir}/${s_base}_mask_resampled.mnc";
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+# &do_cmd( 'mincresample', '-clobber', '-like', $target_masked,
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+# '-nearest_neighbour', '-transform', $opt{init_xfm},
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+# $opt{source_mask}, $mask_resampled );
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+# $opt{source_mask} = $mask_resampled;
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+# }
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+#}
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+
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+$prev_xfm = undef;
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+
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+# a fitting we shall go...
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+for ($i=0; $i<=$#conf; $i++){
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+
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+ # set up intermediate files
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+ $tmp_xfm = "$tmpdir/$s_base\_$i.xfm";
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+ $tmp_source = "$opt{work_dir}/$s_base\_$conf[$i]{blur_fwhm}";
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+ $tmp_target = "$opt{work_dir}/$t_base\_$conf[$i]{blur_fwhm}";
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+
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+ $tmp_source = "$opt{work_dir}/$s_base\_$conf[$i]{blur_fwhm}";
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+ $tmp_target = "$opt{work_dir}/$t_base\_$conf[$i]{blur_fwhm}";
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+
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+ if($opt{close})
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+ {
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+ next if($conf[$i]{blur_fwhm}>8);
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+ $conf[$i]{simplex}=1;
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+ $conf[$i]{steps}=[qw/2 2 2/];
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+ }
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+
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+ print STDOUT "-+-------------------------[$i]-------------------------\n".
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+ " | steps: @{$conf[$i]{steps}}\n".
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+ " | blur_fwhm: $conf[$i]{blur_fwhm}\n".
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+ " | simplex: $conf[$i]{simplex}\n".
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+ " | source: $tmp_source\_$conf[$i]{type}.mnc\n".
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+ " | target: $tmp_target\_$conf[$i]{type}.mnc\n".
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+ " | xfm: $tmp_xfm\n".
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+ "-+-----------------------------------------------------\n".
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+ "\n";
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+
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+ # blur the masked source and target images
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+ my @grad_opt = ();
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+ push( @grad_opt, '-gradient' ) if( $conf[$i]{type} eq "dxyz" );
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+
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+ if($conf[$i]{blur_fwhm}>0) # actually this should be 1
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+ {
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+ if(!-e "$tmp_source\_$conf[$i]{type}.mnc") {
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+ &do_cmd('mincblur', '-clobber', '-no_apodize', '-fwhm', $conf[$i]{blur_fwhm},
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+ @grad_opt, $source_masked, $tmp_source);
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+ }
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+ if(!-e "$tmp_target\_$conf[$i]{type}.mnc") {
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+ &do_cmd('mincblur', '-clobber', '-no_apodize', '-fwhm', $conf[$i]{blur_fwhm},
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+ @grad_opt, $target_masked, $tmp_target);
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+ }
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+ if(defined($opt{sec_source}) && defined($opt{sec_target}) )
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+ {
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+ $tmp_sec_source = "$opt{work_dir}/sec_$s_base\_$conf[$i]{blur_fwhm}";
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+ $tmp_sec_target = "$opt{work_dir}/sec_$t_base\_$conf[$i]{blur_fwhm}";
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+ if(! -e "$tmp_sec_source\_$conf[$i]{type}.mnc")
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+ {
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+ &do_cmd('mincblur', '-clobber', '-no_apodize', '-fwhm',
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+ $conf[$i]{blur_fwhm},
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+ @grad_opt, $opt{sec_source}, $tmp_sec_source);
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+ }
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+
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+ if(! -e "$tmp_sec_target\_$conf[$i]{type}.mnc")
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+ {
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+ &do_cmd('mincblur', '-clobber', '-no_apodize', '-fwhm',
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+ $conf[$i]{blur_fwhm},
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+ @grad_opt, $opt{sec_target}, $tmp_sec_target);
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+ }
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+ $tmp_sec_source="$tmp_sec_source\_$conf[$i]{type}.mnc";
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+ $tmp_sec_target="$tmp_sec_target\_$conf[$i]{type}.mnc";
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+ }
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+ } else { # noop
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+ if(!-e "$tmp_source\_$conf[$i]{type}.mnc") {
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+ do_cmd('ln','-s',$source_masked,"$tmp_source\_$conf[$i]{type}.mnc");
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+ }
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+ if(!-e "$tmp_target\_$conf[$i]{type}.mnc") {
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+ &do_cmd('ln', '-s', $target_masked, "$tmp_target\_$conf[$i]{type}.mnc");
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+ }
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+ if(defined($opt{sec_source}) && defined($opt{sec_target}) )
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+ {
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+ $tmp_sec_source =$opt{sec_source};
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+ $tmp_sec_target =$opt{sec_target};
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+ }
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+ }
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+ # set up registration
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+ @args = ('minctracc', '-clobber', $opt{lsqtype},
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+ '-step', @{$conf[$i]{steps}}, '-simplex', $conf[$i]{simplex},
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+ '-tol', $conf[$i]{tolerance});
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+
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+ # Initial transformation will be computed from the from Principal axis
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+ # transformation (PAT).
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+ push(@args, @{$conf[$i]{trans}}) if( defined $conf[$i]{trans} && !$opt{init_xfm} );
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+
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+ # Current transformation at this step
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+ if( defined $prev_xfm )
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+ {
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+ push(@args, '-transformation', $prev_xfm ) ;
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+ } elsif( defined $opt{init_xfm} ) {
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+ push(@args, '-transformation', $opt{init_xfm} ) ;
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+ #push(@args, '-est_center' ) ;
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+
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+ } elsif($opt{close}) {
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+ push(@args, '-identity') ;
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+ }
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+
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+ # masks (even if the blurred image is masked, it's still preferable
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+ # to use the mask in minctracc)
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+ push(@args, '-source_mask', $opt{source_mask} ) if defined($opt{source_mask});
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+ push(@args, '-model_mask', $opt{target_mask}) if defined($opt{target_mask});
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+
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+ if($tmp_sec_source && $tmp_sec_target )
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+ {
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+ push(@args, $opt{cost_function},
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+ "$tmp_source\_$conf[$i]{type}.mnc",
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+ "$tmp_target\_$conf[$i]{type}.mnc");
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+ push(@args, '-feature_vol',$tmp_sec_source,$tmp_sec_target,'xcorr',1.0);
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+ } else {
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+ push(@args, $opt{cost_function},"$tmp_source\_$conf[$i]{type}.mnc",
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+ "$tmp_target\_$conf[$i]{type}.mnc");
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+ }
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+
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+ push @args,'-w_shear',0,0,0 if $opt{noshear};
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+ push @args,'-w_scales',0,0,0 if $opt{noscale};
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|
|
+ push @args,'-w_translations',0,0,0 if $opt{noshift};
|
|
|
+ push @args,'-w_rotations',0,0,0 if $opt{norot};
|
|
|
+
|
|
|
+ # add files and run registration
|
|
|
+ push(@args, $tmp_xfm);
|
|
|
+ &do_cmd(@args);
|
|
|
+
|
|
|
+ $prev_xfm = $tmp_xfm;
|
|
|
+}
|
|
|
+
|
|
|
+# Concatenate transformations if an initial transformation was given.
|
|
|
+
|
|
|
+#if( defined $opt{init_xfm} ) {
|
|
|
+# &do_cmd( 'xfmconcat', $prev_xfm, $opt{init_xfm}, $outxfm );
|
|
|
+#} else {
|
|
|
+ do_cmd( 'mv', '-f', $prev_xfm, $outxfm );
|
|
|
+#}
|
|
|
+
|
|
|
+# resample if required
|
|
|
+if(defined($outfile)){
|
|
|
+ print STDOUT "-+- creating $outfile using $outxfm\n".
|
|
|
+ &do_cmd( 'mincresample', '-clobber', '-like', $target,
|
|
|
+ '-transformation', $outxfm, $source, $outfile );
|
|
|
+}
|
|
|
+
|
|
|
+sub do_cmd {
|
|
|
+ print STDOUT "@_\n" if $opt{verbose};
|
|
|
+ if(!$opt{fake}){
|
|
|
+ system(@_) == 0 or die;
|
|
|
+ }
|
|
|
+}
|
|
|
+
|
|
|
+linear_registration.sh
|
|
|
+
|
|
|
+bestlinreg_s2 DKT_Atlas.mnc mni_icbm152_t1_tal_nlin_sym_09c.mnc DKT2icbm.xfm --lsq9
|
|
|
+
|
|
|
+resampling.sh
|
|
|
+
|
|
|
+itk_resample DKT_Atlas.mnc --like mni_icbm152_t1_tal_nlin_sym_09c.mnc --transform DKT2icbmnl.xfm DKT_Atlas_icbm_nl.mnc --label --clobber
|
|
|
+
|
|
|
+CerebrA_LabelDetails.csv
|
|
|
+
|
|
|
+Mindboggle ID,Label Name,CerebrA ID,,Notes,Dice Kappa
|
|
|
+,,RH Labels,LH Labels,,
|
|
|
+2002,Caudal Anterior Cingulate,30,81,,0.79
|
|
|
+2003,Caudal Middle Frontal,42,93,Improved distinction from Precentral ,0.73
|
|
|
+2005,Cuneus,43,94,,0.67
|
|
|
+2006,Entorhinal,36,87,Improved delimitation ,0.78
|
|
|
+2007,Fusiform,24,75,,0.77
|
|
|
+2008,Inferior Parietal,10,61,,0.75
|
|
|
+2009,Inferior temporal,3,54,Removed dorsal part MT,0.72
|
|
|
+2010,Isthmus Cingulate,33,84,,0.79
|
|
|
+2011,Lateral Occipital,34,85,,0.76
|
|
|
+2012,Lateral Orbitofrontal,7,58,,0.8
|
|
|
+2013,Lingual,12,63,,0.75
|
|
|
+2014,Medial Orbitofrontal,15,66,,0.72
|
|
|
+2015,Middle Temporal,28,79,Added dorsal part,0.72
|
|
|
+2016,Parahippocampal,18,69,,0.86
|
|
|
+2017,Paracentral,16,67,,0.77
|
|
|
+2018,Pars Opercularis,32,83,,0.77
|
|
|
+2019,Pars Orbitalis,44,95,,0.8
|
|
|
+2020,Pars Triangularis,22,73,,0.76
|
|
|
+2021,Pericalcarine,6,57,,0.6
|
|
|
+2022,Postcentral,13,64,,0.82
|
|
|
+2023,Posterior Cingulate,47,98,,0.8
|
|
|
+2024,Precentral,35,86,,0.84
|
|
|
+2025,Precuneus,31,82,,0.8
|
|
|
+2026,Rostral Anterior Cingulate,8,59,,0.72
|
|
|
+2027,Rostral Middle Frontal,1,52,Improved delimitation from CMF,0.74
|
|
|
+2028,Superior Frontal,38,89,,0.82
|
|
|
+2029,Superior Parietal,9,60,Improved delimitation from Precuneus and IP,0.72
|
|
|
+2030,Superior Temporal,45,96,Added dorsal part limiting with IP and Supramarginal,0.87
|
|
|
+2031,Supramarginal,51,102,,0.81
|
|
|
+2034,Transverse Temporal,14,65,,0.85
|
|
|
+2035,Insula,23,74,,0.88
|
|
|
+16,Brainstem,11,62,"Completed filling, removed labelled voxels out of actual brainstem and removed CWM labels in brainstem area. ",0.65
|
|
|
+14,Third Ventricle,29,80,,0.68
|
|
|
+15,Fourth Ventricle,37,88,Missing label. Manually delimited using CSF threshold. ,0.39
|
|
|
+85,Optic Chiasm,17,68,Almost inexistent label and out of place in original labelling Completed OC and tracts (originally labelled as Ventral Diencephalon),0
|
|
|
+43,Lateral Ventricle,41,92,Improved continuity of labelled voxels,0.89
|
|
|
+44,Inferior Lateral Ventricle,5,56,,0.12
|
|
|
+45,Cerebellum Gray Matter,46,97,"Completed filling using threshold for CGM, removed cerebellum labels out of area (within brainstem and vermis area)",0.83
|
|
|
+46,Cerebellum White Matter,39,90,"Improved according threshold for CWM, removed labels in brainstem and vermis. ",0.73
|
|
|
+49,Thalamus,40,91,,0.97
|
|
|
+50,Caudate,49,100,Completed filling using threshold ,0.84
|
|
|
+51,Putamen,21,72,Corrected uniformity using threshold,0.87
|
|
|
+52,Pallidum,27,78,Improved delimitation between putamen and pallidum,0.83
|
|
|
+53,Hippocampus,48,99,,0.69
|
|
|
+54,Amygdala,19,70,,0.64
|
|
|
+58,Accumbens Area,4,55,,0.76
|
|
|
+60,Ventral Diencephalon,26,77,,0.93
|
|
|
+92,Basal Forebrain,25,76,,0.82
|
|
|
+630,Vermal lobules I-V,50,101,Improved delimitation with other vermal lobules and cerebellar hemispheres,0.66
|
|
|
+631,Vermal lobules VI-VII,2,53,Improved delimitation with other vermal lobules and cerebellar hemispheres,0.38
|
|
|
+632,Vermal lobules VIII-X,20,71,Improved delimitation with other vermal lobules and cerebellar hemispheres,0.44
|