# Anesthesia_CA1

This repository contains data related to the article "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation" (https://www.biorxiv.org/content/10.1101/2020.06.05.135905v1).

We provide calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness, sleep and under various general anesthetics.
Following the structure of the repository, the dataset contains:
- LFP and SUA recordings from 4 mice under Isoflurane, Keta/Xyl and MMF anesthesia (electrophysiology folder)
- 189 distinct 2-photon calcium imaging recordings from 7 different mice under Isoflurane, Keta/Xyl and MMF anesthesia (calcium imaging folder)
- LFP and SUA recordings in awake and sleep conditions (with sleep scoring) from 7 and 4 mice respectively (Sleep Data/LFP and Sleep Data/SUA folders)
- 74 distinct 2-photon calcium imaging recordings from 3 mice in awake and sleep conditions (Sleep Data/calcium imaging folder)

## Data structure info

Most data structure should be self explanatory / inferrable from the description in the manuscript, but some useful information is provided in the respective "meta_data" excel files.

### Miscellaneous

- information about the numpy calcium imaging files can be found in the suite2p documentation page (https://suite2p.readthedocs.io/en/latest/)
- for LFP data, only the CA1 reversal channel is provided
- LFP data has a sampling rate of 320 Hz and has been band-pass filtered in the 0.1-160 Hz range
- code used for analysis/stats and stats dataset can be found at https://github.com/mchini/Yang_Chini_et_al and https://github.com/OpatzLab/HanganuOpatzToolbox