Calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness and under various general anesthetics

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Andrey Formozov 8a784cc017 ORCID and references		2 years ago
Anesthesia	1d24963b3f gin commit from andrey-UX510UXK	3 years ago
Sleep	a48f286f93 gin commit from andrey-UX510UXK	2 years ago
data_colab	7985671ffc gin commit from andrey-UX510UXK	2 years ago
meta_data	d50601e98b gin commit from andrey-UX510UXK	2 years ago
repo_figures	e94819395d repo_figures added for gifs	2 years ago
validation	659436b2f9 gin commit from andrey-UX510UXK	2 years ago
LICENSE	69d0ea48c9 Initial commit	4 years ago
README.md	6b2ab4b5ab updated article info	2 years ago
datacite.yml	8a784cc017 ORCID and references	2 years ago

Anesthesia_CA1

This repository contains data related to the article "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation" (https://www.biorxiv.org/content/10.1101/2020.06.05.135905v1). Published in PLOS Biology doi: 10.1371/journal.pbio.3001146.

We provide calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness, sleep and under various general anesthetics (Isoflurane, Keta/Xyl and MMF).

Awake	Isoflurane	Keta/Xyl	MMF

Following the structure of the repository, the dataset contains:

LFP and SUA recordings from 4 mice under Isoflurane, Keta/Xyl and MMF anesthesia (electrophysiology folder)
189 distinct 2-photon calcium imaging recordings from 7 different mice under Isoflurane, Keta/Xyl and MMF anesthesia (calcium imaging folder)
LFP and SUA recordings in awake and sleep conditions (with sleep scoring) from 7 and 4 mice respectively (Sleep Data/LFP and Sleep Data/SUA folders)
74 distinct 2-photon calcium imaging recordings from 3 mice in awake and sleep conditions (Sleep Data/calcium imaging folder)

This repository contains electrophysiological data and processed calcium imaging recordings. Raw calcium imaging recordings are available in another repository.

Data structure info

Most data structure should be self explanatory / inferrable from the description in the manuscript, but some useful information is provided in the respective "meta_data" excel files.

Miscellaneous

information about the numpy calcium imaging files can be found in the suite2p documentation page (https://suite2p.readthedocs.io/en/latest/)
for LFP data, only the CA1 reversal channel is provided
LFP data has a sampling rate of 320 Hz and has been band-pass filtered in the 0.1-160 Hz range
code used for the analysis and statistics together with stats-dataset can be found at https://github.com/mchini/Yang_Chini_et_al and https://github.com/OpatzLab/HanganuOpatzToolbox

datacite.yml
Title	Calcium imaging and electrophysiology of hippocampal activity under anesthesia and natural sleep in mice
Authors	Formozov,Andrey;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0001-8935-8612 Chini,Mattia;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-5782-9720 Dieter,Alexander;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-9154-4833 Yang,Wei;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-1052-8808 Pöpplau,Jastyn A.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-4350-3164 Hanganu-Opatz,Ileana L.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-4787-1765 Wiegert,J. Simon;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0003-0893-9349
Description	Large-scale, annotated collection of 2-photon calcium imaging data and electrophysiological recordings in CA1 of the murine hippocampus under three distinct anesthetics (Isoflurane, Keta/Xyl and MMF), during natural sleep, and during wakefulness.
License	Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References	Formozov, A. et al. Calcium imaging and electrophysiology of hippocampal activity under anesthesia and natural sleep in mice. Scientific data. 2022 [] (IsDescribedBy) Yang, W., Chini, M. et al. Anesthetics fragment hippocampal network activity, alter spine dynamics, and affect memory consolidation. PLoS biology, 19(4), e3001146 [doi:10.1371/journal.pbio.3001146] (IsSupplementTo)
Funding	German Research Council, SFB936/B8 German Research Council, SFB936/B5 European Research Council, ERC-2016-StG-714762 European Research Council, ERC-2015-CoG-681577 Alexander von Humboldt Foundation, Research Fellowship EU-project euSNN, MSCA-ITN-H2020-860563 LandesforschungsfÃ¶rderung Hamburg, LFF76 and LFF73 Chinese Scholarship Council, CSC 201606210129
Keywords	Neuroscience Anesthesia Anaesthesia Sleep Recovery Hippocampus CA1 Pyramidal neurons Calcium imaging data Electrophysiological recordings Pupillometry EMG data
Resource Type	Dataset

README.md

Anesthesia_CA1

Data structure info

Miscellaneous