2023_Blaschke_Stroke_tDCS_rsfMRI/readme.md

Accompaning Dataset: Blaschke et al., Stroke 2023

doi: 10.1161/STROKEAHA.123.042808

Transcranial Direct Current Stimulation Reverses Stroke-Induced Network Alterations in Mice.

This is a dataset of longitudinal MRI (T2w and rs-fMRI) in mice after cortical stroke (photothrombosis) for up to four weeks and early cathodal tDCS compared to sham stimulation and healthy controls.

• All inputs (MRI raw and processed, Behavior data) are located in inputs/.

• All custom code is located in code/.

• All of the final results (of any kind) is located in results/.

Abstract:

Background: Beyond focal effects, stroke lesions impact the function of distributed networks. We here investigated (1) whether transcranial direct current stimulation (tDCS) alters the network changes induced by cerebral ischemia and (2) whether functional network parameters predict the therapeutic efficacy of tDCS in a mouse model of focal photothrombotic stroke.

Methods: Starting 3 days after stroke, cathodal tDCS (charge density=39.6 kC/m²) was applied over 10 days in male C57Bl/6J mice under light anesthesia over the lesioned sensory-motor cortex. Functional connectivity (resting-state functional magnetic resonance imaging) was evaluated for up to 28-day poststroke, with global graph parameters of network integration computed.

Results: Ischemia induced a subacute increase in connectivity accompanied by a significant reduction in characteristic path length, reversed by 10 days of tDCS. Early measures of functional network alterations and the network configuration at prestroke baseline predicted spontaneous and tDCS-augmented motor recovery.

Discussion: Stroke induces characteristic network changes throughout the brain that can be detected by resting-state functional magnetic resonance imaging. These network changes were, at least in part, reversed by tDCS. Moreover, early markers of a network impairment and the network configuration before the insult improve the prediction of motor recovery.

Keywords

• animals
• brain
• ischemia
• magnetic resonance imaging
• mice
• functional connectivity
• transcranial direct current stimulation