cff-version: 1.2.0
title: >-
  GCAMP7 live imaging of AP-2KO mice
message: This is our project description
type: dataset
authors:
  - given-names: Marianna
    family-names: Tolve
    email: marianna.tolve@uk-koeln.de
    orcid: https://orcid.org/0000-0002-0893-2967
    affiliation: >-
      University of Cologne, Medical Faculty, Center for Physiology & CECAD
  - given-names: Natalia
    family-names: Kononenko
    email: natalia.kononenko@uk-koeln.de
    affiliation: >-
      Cologne Excellence Cluster Cellular Stress Response in
      Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany;
      Center for Physiology and Pathophysiology, Faculty of Medicine and
      University Hospital Cologne, University of Cologne, Cologne, Germany
    orcid: https://orcid.org/0000-0002-3425-6659
abstract: >-
  The current research proposal focuses on cellular and molecular
  mechanisms underlying the maintenance of voluntary motor control by
  neurons in cerebellum and basal ganglia in mice and how endocytic
  protein AP-2 control this process. This dataset contains live
  imaging data of activity-dependent intracellular Ca2+ dynamics in
  cerebellar organotypic slices of WT and AP-2KO mice. The slices were
  transduced with GCAMP7-expressing AAVs and imaged after 3 weeks in
  vitro with confocal microscopy for 60 seconds. To induce neuronal
  activity, the slice were electrically stimulated at 100Hz on the
  20th second.