# -*- coding: utf-8 -*- from neo.io.basefromrawio import BaseFromRaw from neo.rawio.axonrawio import AxonRawIO from neo.core import Block, Segment, AnalogSignal, Event import quantities as pq class AxonIO(AxonRawIO, BaseFromRaw): """ Class for reading data from pCLAMP and AxoScope files (.abf version 1 and 2), developed by Molecular device/Axon technologies. - abf = Axon binary file - atf is a text file based format from axon that could be read by AsciiIO (but this file is less efficient.) Here an important note from erikli@github for user who want to get the : With Axon ABF2 files, the information that you need to recapitulate the original stimulus waveform (both digital and analog) is contained in multiple places. - `AxonIO._axon_info['protocol']` -- things like number of samples in episode - `AxonIO.axon_info['section']['ADCSection']` | `AxonIO.axon_info['section']['DACSection']` -- things about the number of channels and channel properties - `AxonIO._axon_info['protocol']['nActiveDACChannel']` -- bitmask specifying which DACs are actually active - `AxonIO._axon_info['protocol']['nDigitalEnable']` -- bitmask specifying which set of Epoch timings should be used to specify the duration of digital outputs - `AxonIO._axon_info['dictEpochInfoPerDAC']` -- dict of dict. First index is DAC channel and second index is Epoch number (i.e. information about Epoch A in Channel 2 would be in `AxonIO._axon_info['dictEpochInfoPerDAC'][2][0]`) - `AxonIO._axon_info['EpochInfo']` -- list of dicts containing information about each Epoch's digital out pattern. Digital out is a bitmask with least significant bit corresponding to Digital Out 0 - `AxonIO._axon_info['listDACInfo']` -- information about DAC name, scale factor, holding level, etc - `AxonIO._t_starts` -- start time of each sweep in a unified time basis - `AxonIO._sampling_rate` The current AxonIO.read_protocol() method utilizes a subset of these. In particular I know it doesn't consider `nDigitalEnable`, `EpochInfo`, or `nActiveDACChannel` and it doesn't account for different types of Epochs offered by Clampex/pClamp other than discrete steps (such as ramp, pulse train, etc and encoded by `nEpochType` in the EpochInfoPerDAC section). I'm currently parsing a superset of the properties used by read_protocol() in my analysis scripts, but that code still doesn't parse the full information and isn't in a state where it could be committed and I can't currently prioritize putting together all the code that would parse the full set of data. The `AxonIO._axon_info['EpochInfo']` section doesn't currently exist. """ _prefered_signal_group_mode = 'split-all' def __init__(self, filename): AxonRawIO.__init__(self, filename=filename) BaseFromRaw.__init__(self, filename) def read_protocol(self): """ Read the protocol waveform of the file, if present; function works with ABF2 only. Protocols can be reconstructed from the ABF1 header. Returns: list of segments (one for every episode) with list of analog signls (one for every DAC). """ sigs_by_segments, sig_names, sig_units = self.read_raw_protocol() segments = [] for seg_index, sigs in enumerate(sigs_by_segments): seg = Segment(index=seg_index) t_start = self._t_starts[seg_index] * pq.s for c, sig in enumerate(sigs): ana_sig = AnalogSignal(sig, sampling_rate=self._sampling_rate * pq.Hz, t_start=t_start, name=sig_names[c], units=sig_units[c]) seg.analogsignals.append(ana_sig) segments.append(seg) return segments