Calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness and under various general anesthetics

Andrey Formozov 37d24ca80c Update 'README.md' 5 months ago
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README.md

Anesthesia_CA1

This repository contains data related to the article "Anesthetics fragment hippocampal network activity, alter spine dynamics and affect memory consolidation" (https://www.biorxiv.org/content/10.1101/2020.06.05.135905v1). Published in PLOS Biology doi: 10.1371/journal.pbio.3001146.

We provide calcium imaging and electrophysiology data from hippocampal CA1 during wakefulness, sleep and under various general anesthetics (Isoflurane, Keta/Xyl and MMF).

Awake Isoflurane Keta/Xyl MMF

Following the structure of the repository, the dataset contains:

  • LFP and SUA recordings from 4 mice under Isoflurane, Keta/Xyl and MMF anesthesia (electrophysiology folder)
  • 189 distinct 2-photon calcium imaging recordings from 7 different mice under Isoflurane, Keta/Xyl and MMF anesthesia (calcium imaging folder)
  • LFP and SUA recordings in awake and sleep conditions (with sleep scoring) from 4 and 3 mice, respectively (Sleep Data/LFP and Sleep Data/SUA folders), 1-2 hours of duration.
  • 74 distinct 2-photon calcium imaging recordings from 3 mice in awake and sleep conditions (Sleep Data/calcium imaging folder)

This repository contains electrophysiological data and processed calcium imaging recordings. Raw calcium imaging recordings are available in another repository.

Data structure info

Most data structure should be self explanatory / inferrable from the description in the manuscript, but some useful information is provided in the respective "meta_data" excel files.

Miscellaneous

Typos and bugs reported by users

  • In Fig. 1 and Table 2 of the data descriptor, the duration of the electrophysiological recordings is indicated as fixed to 1.5 hours, while in reality, it varied from recording to recording in different conditions.
datacite.yml
Title Calcium imaging and electrophysiology of hippocampal activity under anesthesia and natural sleep in mice
Authors Formozov,Andrey;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0001-8935-8612
Chini,Mattia;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-5782-9720
Dieter,Alexander;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-9154-4833
Yang,Wei;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-1052-8808
Pöpplau,Jastyn A.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-4350-3164
Hanganu-Opatz,Ileana L.;Institute of Developmental Neurophysiology, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0002-4787-1765
Wiegert,J. Simon;Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany;ORCID:0000-0003-0893-9349
Description Large-scale, annotated collection of 2-photon calcium imaging data and electrophysiological recordings in CA1 of the murine hippocampus under three distinct anesthetics (Isoflurane, Keta/Xyl and MMF), during natural sleep, and during wakefulness.
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References Formozov, A., Chini, M., Dieter, A. et al. Calcium Imaging and Electrophysiology of hippocampal Activity under Anesthesia and natural Sleep in Mice. Sci Data 9, 113 (2022). https://doi.org/10.1038/s41597-022-01244-2 [https://doi.org/10.1038/s41597-022-01244-2] (IsDescribedBy)
Yang, W., Chini, M. et al. Anesthetics fragment hippocampal network activity, alter spine dynamics, and affect memory consolidation. PLoS biology, 19(4), e3001146. https://doi.org/10.1371/journal.pbio.3001146 [doi:10.1371/journal.pbio.3001146] (IsSupplementTo)
Formozov A., Chini M., Dieter A., Yang W., Pöpplau J.A., Hanganu-Opatz I.L., Wiegert J.S. (2022) Calcium imaging of hippocampal activity under anesthesia and natural sleep in mice. G-Node. https://doi.org/10.12751/g-node.s549qk [doi:10.12751/g-node.s549qk] (IsDerivedFrom)
Funding German Research Council, SFB936/B8
German Research Council, SFB936/B5
European Research Council, ERC-2016-StG-714762
European Research Council, ERC-2015-CoG-681577
Alexander von Humboldt Foundation, Research Fellowship
EU-project euSNN, MSCA-ITN-H2020-860563
Landesforschungsförderung Hamburg, LFF76 and LFF73
Chinese Scholarship Council, CSC 201606210129
Keywords Neuroscience
Anesthesia
Anaesthesia
Sleep
Recovery
Hippocampus
CA1
Pyramidal neurons
Calcium imaging data
Electrophysiological recordings
Pupillometry
EMG data
Resource Type Dataset