Manuel Schottdorf
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README.md
Details of the optics
This folder contains details of the optics. An explicit simulation of the optical path in Zemax is provided in the file AchromatAndFold.ZMX. The achromatic lens is stock #47-699 from Edmund Optics.
This is a sketch of the optical path:
Caption: Light Path of the Stamp Setup: S - stamp, M - MEA, P - folding prism, A - aperture stop (optional), L- lens, C-Camera. The detail shows how stamp faces in contact (b) can be clearly stinguished from raised areas of the stamp (a) or faces not yet in contact (c).
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| Title | Construction and use of an accurate positioning-µCP device |
| Authors |
Samhaber,Robert;Max-Planck-Institute for Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany; Max Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany; Bernstein Center for Computational Neuroscience, Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany; SFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany
Schottdorf,Manuel;Max-Planck-Institute for Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany; Max Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany; Bernstein Center for Computational Neuroscience, Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany;ORCID:0000-0002-5468-4255 El Hady,Ahmed;Max-Planck-Institute for Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany; Max Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany; Bernstein Center for Computational Neuroscience, Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany; SFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany;ORCID:0000-0002-0045-8017 Bröking,Kai;Max-Planck-Institute for Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany; Max Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany; Bernstein Center for Computational Neuroscience, Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany Daus,Andreas;Faculty of Engineering, University of Applied Science, Würzburger Straße 45, 63743 Aschaffenburg, Germany Thielemann,Christiane;Faculty of Engineering, University of Applied Science, Würzburger Straße 45, 63743 Aschaffenburg, Germany Stühmer,Walter;Max-Planck-Institute for Experimental Medicine, Dept. Molecular Biology of Neuronal Signals, Hermann-Rein-Str. 3, 37075 Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany Wolf,Fred;Max Planck Institute for Dynamics and Self-Organization, Dept. Nonlinear Dynamics, Am Faßberg 17, 37077 Göttingen, Germany; Bernstein Center for Computational Neuroscience, Göttingen, Germany; Bernstein Focus Neurotechnology, Göttingen, Germany; SFB-889 Cellular Mechanisms of Sensory Processing, Göttingen, Germany; Faculty of Physics, Georg-August-Universität Göttingen, Göttingen, Germany |
| Description | This repository accompanies the manuscript Samhaber et al. J Neurosc Methods 2016. It contains detailed instructions for building a micro contact printing (μCP) machine and for using it to spatially structure neuronal cultures. Its purpose is to allow any lab without access to a lithography facility to structure cultures of neurons with the method developed in our group. It contains details of the preparation of neuronal cultures, all protocols used, and the construction of the μCP machine including all CAD files. |
| License | Creative Commons Attribution 4.0 International Public License (https://creativecommons.org/licenses/by/4.0/) |
| References |
Samhaber et al. Growing neuronal islands on multi-electrode arrays using an accurate positioning-μCP device [doi:10.1016/j.jneumeth.2015.09.022] (IsSupplementTo)
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| Funding |
BMBF, 01GQ0811
BMBF, 01GQ01005B BMBF, 01GQ0922 ZIM, KF2710201 DF0 DFG, SFB889 DFG, Cluster of Excellence: Nanoscale Microscopy and Molecular Physiology of the Brain. VolkswagenStiftung, ZN2632 Boehringer Ingelheim Fonds |
| Keywords |
Neuroscience
Electrophysiology µCP MEA Patterning |
| Resource Type |
Dataset |