Chronic in vivo calcium imaging dataset of neuronal populations in the auditory cortex of passively listening mice. Baseline and auditory cued fear conditioning cohorts.

Dominik Aschauer de8cbbaca3 'datacite.yml' ändern 2 years ago
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README.md

Aschauer_invivo_chronic_calcium_imaging_auditorycortex

Chronic in vivo calcium imaging dataset of neuronal populations in the auditory cortex of passively listening mice. Baseline and auditory cued fear conditioning cohorts. Each filename (e.g. 'mouse01_fov01_day01.mat') describes the index of the imaged mouse, the respective index of the fov, and the imaging day. In each file, you can find the images of both channels (channel1 = H2B::mCherry, channel2 = GCaMP6m) as a three dimensional matrix of 156 (pixel number of rows), 256 (pixel number of columns), and the number of frames. Additionally, we provide the information about sensory stimulations in the file 'stimulation_info.zip'. Here, the individual filename matches the filename of the image files. In each file (e.g. 'mouse01_fov01_day01.mat'), you can find a vector of the length of the number of frames in the respective imaging session. The value in the vector indicates which and when a stimulus was presented (0 = no stimulus, 1-34 = stimulus index). The information on which stimulus index was which stimulus can be found in the file 'stimulus_names.mat'). Additionally, we provide the information about extracted rois after image processing in the file 'roi_coordinates.zip'. In each file (e.g. 'mouse01_fov01_day01.mat'), you can find a three dimensional matrix of two spatial dimensions, roi index, and the number of frames. The code for image processing can be downloaded from a public repository (DOI: 10.5281/zenodo.5822486). Further information about analysis of this dataset can be found in the corresponding published article.

datacite.yml
Title Chronic in vivo calcium imaging dataset of neuronal populations in the auditory cortex of passively listening mice. Baseline and auditory cued fear conditioning cohorts.
Authors Aschauer,Dominik Florian;JGU Mainz;ORCID:0000-0002-2600-999X
Eppler,Jens-Bastian;FIAS Frankfurt;ORCID:0000-0003-4288-0918
Description In this repository is a dataset of chronically imaged neuronal populations in the auditory cortex of mice being exposed to a set of short sound stimuli. The imaging was performed on four timepoints two days apart each over the course of seven days. Two different experimental conditions, one cohort was imaged under baseline conditions without any behavioral manipulations. The other cohort was imaged with an auditory cued fear conditioning session in between the second and third imaging time point. Image time series are two-channel images, channel one is histone fused mCherry (red fluorescent protein), H2B::mCherry. Channel two is GCaMP6m, green fluorescent genetically encoded calcium indicator. Additionally, we provide the information about auditory sensory stimulations in the file 'stimulation_info.zip'. Additionally, we provide the information about extracted rois after image processing in the file 'roi_coordinates.zip'. ROIs were aligned using a software package for image processing which can be downloaded from a public repository (DOI: 10.5281/zenodo.5822486). Further information about analysis of this dataset can be found in the corresponding published article.
License Creative Commons CC0 1.0 Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/)
References Eppler, Jens-Bastian. (2022). 2-photon data preprocessing pipeline (1.0). Zenodo. [doi:10.5281/zenodo.5822486] (IsSupplementTo)
Funding
Keywords Neuroscience
Auditory
Mouse
Two-Photon
Calcium Imaging
Chronic
Basal Conditions
Fear Conditioning
Learning
Resource Type Dataset