Date: 2021-02-11
Experiment: WT DREADD awake DG + CA3
Experimenter: Mariela
Objective: in awake
	4x16 probe in DG
	32 linear 25 um probe in CA3 at 20 deg angle

SURGERIES

Mouse : 4496 - 1 dot , DOB 16-12-2020 , 23.4 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
Solutions: ACSF 2018


10:15	iso induction + bupre 
11:05	Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5), 
	CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.72;0.60). 
11:50	craniotomy DG = ok, CA3 ok 
12:15	Recovery 


RECORDINGS


Mouse : 4496 - 1 dot , DOB 16-12-2020 , 23.4 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 

13:45	Removed the silicon, OK
14:05	zero S404 in the middle of the DG craniotomy. some bleeding 
	zero CCO5 in the middle of the CA3 craniotomy
	take impedances
	S404 (0.01;-0.08,-2.21)
14:50	CA1
	1310-1550 CA1 all shanks (shank 4 is the latest to reach CA 1)
	SWR go up above the layer, and down below CA1. 
	DS go down above the layer and up in the 2/3rd  of the layer itself
	We stop as soon as we hear spike, because they are most likely from CA3
	All deepst channel to be up and all the shallow to be down
	Heard spikes 
	2110 - shanks 1, 2 and 3
	Saw dentate spike reversal : yes 
15:40 	CCO5 (0;0;-2.790)
	1490- 1750 CA1
	2500-2790 CA3
	we stopped at -2.79
	There is noise, ears or hair touching probe or head bar???? check grounding of the probe???
16:30	Recording 1
17:00	Inject 200uL CNO, then recording 2
17:30 	Recording 3
18:15	remove probes	
18:30	ket/xyl 0.35ml IP - sac

CC05 had noise during several moments of the recordings (hair/ear touching the probe?)

Notes for histology:
4406
CA3: 20 deg, DiI (1*1 marks total)
DG: 0 deg, DiI x (1*4 marks total)
TdTomato