Date: 2021-02-10
Experiment: WT DREADD awake DG + CA3
Experimenter: Mariela
Objective: in awake
	4x16 probe in DG
	32 linear 25 um probe in CA3 at 20 deg angle

SURGERIES

Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 
Solutions: ACSF 2018


10:40	iso induction + bupre 
11:05	Injection site = (0,0,0) ==> DG marks (-0.75;0.5), (0.25;-0.5), 
	CA3 marks at (1.25;-0.25), (0.25,1.25) ==> center (0.92;0.63). 
12:20	craniotomy DG = oedema from the injection scar, CA3 ok 
12:25	Recovery 


RECORDINGS


Mouse : 4497 - 2 dots , DOB 16-12-2020 , 22.8 g
injection: LV-160 / pLenti225.CamKII.hM4D(Gi)-mCherry 

14:00	Removed the silicon, OK
14:35	zero S404 in the middle of the DG craniotomy 
	zero CCO5 in the middle of the CA3 craniotomy
	take impedances
	S404 (0;0,-2.25)
15:15	CA1
	1370-1500 CA1 all shanks
	SWR go up above the layer, and down below CA1. 
	DS go down above the layer and up in the 2/3rd  of the layer itself
	CHANGED approached about DG: as previous experiments were too lareral and too deep
	we stop as soon as we hear spike, because they are most likely from CA3
	All deepst channel to be up and all the shallow to be down
	Heard spikes 
	2150 - shank 3 and 4
	Saw dentate spike reversal : yes 
16:10 	CCO5 (0;0;-2.78)
	1570-1950 CA1
	2600-2785 CA3
	we stopped at -2.78
17:22	Recording 1
17:55	Inject 200uL saline, then recording 2
18:25 	Recording 3
19:10	remove probes	
19:20	Iso - sac

CC05 had noise during several moments of the recordings (hair touchong the probe?)

Notes for histology:
4406
CA3: 20 deg, DiI (1*1 marks total)
DG: 0 deg, DiI x (1*4 marks total)
TdTomato